Artificial soil microcosms: a tool for studying microbial autecology under controlled conditions.

نویسنده

  • Richard J Ellis
چکیده

A novel artificial microcosm containing all the essential chemical components of soil, but with reduced heterogeneity and biological complexity, has been developed. Its utility for supporting realistic microbial populations was demonstrated and an example of how competing bacteria can be studied is illustrated.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Harsh summer conditions caused structural and specific functional changes of microbial communities in an arable soil

The mineralization of the herbicide 3-(4-isopropylphenyl)-1,1-dimethylurea (isoproturon) was reduced after the dry and hot summer 2003 in a soil profile placed in a field lysimeter. A different isoproturon mineralization pattern remained in soil material taken at two different soil depths (0–5 cm and 15–20 cm), although soil material was re-equilibrated at adequate climatic conditions. Special ...

متن کامل

Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm.

Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed w...

متن کامل

Detection of genes involved in biodegradation and biotransformation in microbial communities by using 50-mer oligonucleotide microarrays.

To effectively monitor biodegrading populations, a comprehensive 50-mer-based oligonucleotide microarray was developed based on most of the 2,402 known genes and pathways involved in biodegradation and metal resistance. This array contained 1,662 unique and group-specific probes with <85% similarity to their nontarget sequences. Based on artificial probes, our results showed that under hybridiz...

متن کامل

Gene transfer in soil systems using microcosms

Soil microcosms are a valuable tool for the study of genetic interactions between microorganisms in natural soil environments. However, many factors, some of which are better studied than others, can affect gene transfer events occurring in soil systems, and hence should be carefully considered when performing experiments in soil microcosms. This paper is a guide to setting up and evaluating ge...

متن کامل

Degradation rates of aged petroleum hydrocarbons are likely to be mass transfer dependent in the field.

Evidence for on site biodegradation may be difficult to provide at heterogeneous sites without additional experiments in controlled laboratory conditions. In this study, microbial activities measured as CO2 and CH4 production were compared in situ, in intact soil cores and in bottle microcosms containing sieved soils. In addition, biodegradation rates were determined by measuring the decrease i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of microbiological methods

دوره 56 2  شماره 

صفحات  -

تاریخ انتشار 2004